anti tubulin 11h10 rabbit mab  (Cell Signaling Technology Inc)

Journal: Journal of Crohn's & Colitis

Article Title: Epithelial genetic muscarinic receptor 3 ablation induces sex-specific modulation of colonic intestinal progenitor cells and response to intestinal injury

doi: 10.1093/ecco-jcc/jjaf038

Figure Lengend Snippet: Colonic epithelial M3R ablation leads to tuft cell expansion and increased acetylcholine production. ( a ) ACh levels were measured by ELISA in colonic tissue harvested from WT and Vil.M3R mice. ( b ) Representative images of p-EGFR immunostaining of colon harvested from WT and Vil.M3R mice with red arrows showing p-EGFR + cells. Scale bar = 50 µm (40×). p-EGFR positive tuft cells were counted along colonic crypts. ( c ) Western blot was performed from colonic protein extracts from WT and Vil.M3R animals with respective antibodies. Relative protein expression of PI3K was calculated compared to α-Tubulin. ( d ) Heatmap of the average abundance of phosphorylated proteins involved in PI3K/Akt pathway in colonic protein extracts from male and female WT and Vil.M3R mice. Statistical differences were detected by 2way ANOVA with Šídák’s multiple comparisons test. Results are shown as truncated violin plots with median represented as dashed line. * P < .05, ** P < .01.

Article Snippet: α-Tubulin (11H10) , Rabbit , Cell Signaling , 1:1000 (WB).

Techniques: Enzyme-linked Immunosorbent Assay, Immunostaining, Western Blot, Expressing

Journal: PLOS One

Article Title: The impact of Connexin 43 deficiency on the cell shape and cytoskeleton of murine Sertoli cells: A house with ramshackle walls?

doi: 10.1371/journal.pone.0321292

Figure Lengend Snippet: Antibodies used for immunohistochemistry, immunofluorescence and immunogoldlabelling.

Article Snippet: α-tubulin (11H10) Rabbit mAb , Cell Signaling Technology , 1:4000 , sodium citrate buffer (pH 6.0) , anti-rabbit En Vision; DAKO, Hamburg, Germany.

Techniques: Immunohistochemistry, Immunofluorescence

Journal: PLOS One

Article Title: The impact of Connexin 43 deficiency on the cell shape and cytoskeleton of murine Sertoli cells: A house with ramshackle walls?

doi: 10.1371/journal.pone.0321292

Figure Lengend Snippet: Seminiferous tubules of adult wild type mice (A, C) show a stage-specific spoke-like staining pattern similar to what is known for rats . The radial staining pattern consists of band- or cable-like structures extending from the tubular base to the lumen (arrow). In some epithelial stages, the band-like structures branch out in apical Sertoli cell regions and surround spermatids (C). In contrast, Cx43 deficient Sertoli cells (B, D) show a rather diffuse cytoplasmic staining pattern of α-tubulin or many, tightly packed cable-like-structures that are laterally distracted and not in line with the lumen (arrowhead); scale bars = 20 µm.

Article Snippet: α-tubulin (11H10) Rabbit mAb , Cell Signaling Technology , 1:4000 , sodium citrate buffer (pH 6.0) , anti-rabbit En Vision; DAKO, Hamburg, Germany.

Techniques: Staining